Journal: bioRxiv

Article Title: Nanopore amplicon sequencing reveals molecular convergence and local adaptation of opsin genes

doi: 10.1101/2020.06.13.150334

Figure Lengend Snippet: Summary of steps for amplicon sequencing and bioinformatic analyses. Boxes on the left represent individual steps, color-coded based on their phase: red represents sample preparation, yellow represents nanopore sequencing, green represents sample demultiplexing, blue represents read mapping, and purple represents genotyping and analysis. Larger boxes to the right show additional information for each of the steps: the simplified mechanisms by which amplicons are generated and barcoded (top); frequency histogram with read length on the x-axis and number of reads in the y-axis (middle); and how reads are mapped to the reference genome (bottom).

Article Snippet: We examined a potential case of adaptation to novel visual environments in a species flock (Great Lakes salmonids, genus Coregonus ) using a new amplicon genotyping protocol on the Oxford Nanopore Flongle.

Techniques: Amplification, Sequencing, Sample Prep, Nanopore Sequencing, Generated